Establishment of an indirect ELISA method for detecting canine distemper virus antibodies
Abstract
Canine distemper virus (CDV) can cause acute infectious diseases in various carnivorous animals. This study aims to develop an indirect ELISA method for CDV antibody detecting.
Using the purified recombinant CDV antigens as coating agents, and after optimizing the conditions, a preliminary ELISA detection method for CDV specific antibodies was established. The optimal coating concentration of recombinant CDV antigen at 2,0 μg/mL 100 μL/well, overnight coating at 4 °C, blocking at 4 °C for 4 h, serum dilution at 1: 200 and incubation at 37 °C for 1,5 h or 2 h, or 4 °C overnight. No cross-reactivity was observed with CPIV, CAV-1, and CCV antibody-positive serum, indicating good specificity. Both intra-batch and inter-batch CVs were below 5 %, and the detection sensitivity reached 1:500.
About the Authors
Li Laiqing
Belarusian State Technological University ; Guangzhou Youdi Bio-technology Co., Ltd.
Belarus
Belarus
Chairman of the board
Minsk
Guangzhou
Chen Cuicui
Belarusian State Technological University
Belarus
Belarus
Project manager
Minsk
Liang Huankun
Belarusian State Technological University ; Guangzhou Youdi Bio-technology Co., Ltd.
Belarus
Belarus
Quality manager
Minsk
Guangzhou
Shuhai Zhong
Belarusian State Technological University ; Guangzhou Youdi Bio-technology Co., Ltd.
Belarus
Belarus
Head of research and development department
Minsk
Guangzhou
V. N. Leontiev
Belarusian State Technological University
Belarus
Belarus
Candidate of technical sciences
Minsk
I. V. Voitov
Belarusian State Technological University
Belarus
Belarus
Doctor of technical sciences, professor
Minsk
References
1. Martella V., Elia G., Buonavoglia C.. Canine distemper virus. Vet Clin North Am Small Anim Pract. 2008;38(4):787-97, vii-viii. doi: 10.1016/j.cvsm.2008.02.007.
2. Rendon-Marin S., da Fontoura Budaszewski R., Canal C.W., Ruiz-Saenz J. Tropism and molecular pathogenesis of canine distemper virus. Virol J. 2019;16(1):30. doi: 10.1186/s12985-019-1136-6.
3. Loots A.K., Mitchell E., Dalton D.L., Kotzé A., Venter E.H. Advances in canine distemper virus pathogenesis research: a wildlife perspective. J Gen Virol. 2017;98(3):311-321. doi: 10.1099/jgv.0.000666.
4. Woo G.H., Jho Y.S., Bak E.J. Canine distemper virus infection in fennec fox (Vulpes zerda). J Vet Med Sci. 2010 Aug;72(8):1075-9. doi: 10.1292/jvms.09-0510.4.
5. Stancu A.C., Voia O.S., Boldura O.M., Pasca S.A., Luca I., Hulea A.S., Ivan O.R., Dragoescu A.A., Lungu B.C., Hutu I. Unusual Canine Distemper Virus Infection in Captive Raccoons (Procyon lotor). Viruses. 2023;15(7):1536. doi: 10.3390/v15071536.
6. Feng N., Yu Y., Wang T., Wilker P., Wang J., Li Y., Sun Z., Gao Y., Xia X. Fatal canine distemper virus infection of giant pandas in China. Sci Rep. 2016;6:27518. doi: 10.1038/srep27518.5.
7. Lin A.V. Direct ELISA. Methods Mol Biol. 2015;1318:61-7. doi: 10.1007/978-1-4939-2742-5_6.
8. Chen C.C., Lai H.R., Liang H.K. [et al.]. Establishment and preliminary application of timeresolved immunofluorescence analysis method for canine distemper virus. Chinese Journal of Preventive Veterinary Medicine, 2020, 42(3): 258-262.
9. Beineke A., Puff C., Seehusen F., Baumgärtner W. Pathogenesis and immunopathology of systemic and nervous canine distemper. Vet Immunol Immunopathol. 2009;127(1-2):1-18. doi: 10.1016/j.vetimm.2008.09.023.
10. Wang J., Wang J., Li R., Liu L., Yuan W. Rapid and sensitive detection of canine distemper virus by real-time reverse transcription recombinase polymerase amplification. BMC Vet Res. 2017;13(1):241. doi: 10.1186/s12917-017-1180-7.
11. Sarchahi A. A., Arbabi M., Mohebalian H. Detection of canine distemper virus in cerebrospinal fluid, whole blood and mucosal specimens of dogs with distemper using RT-PCR and immunochromatographic assays. Vet Med Sci. 2022;8(4):1390-1399. doi: 10.1002/vms3.790.
12. Knight A.R., Taylor E.L., Lukaszewski R., Jensen K.T., Jones H.E., Carré J.E., Isupov M.N., Littlechild J.A., Bailey S.J., Brewer E., McDonald T.J., Pitt A.R., Spickett C.M., Winyard P.G. A highsensitivity electrochemiluminescence-based ELISA for the measurement of the oxidative stress biomarker, 3-nitrotyrosine, in human blood serum and cells. Free Radic Biol Med. 2018;120:246-254. doi: 10.1016/j.freeradbiomed.2018.03.026.
13. Tan Y., Pang W., Jia X., Zhao M.H. Comparison of the performance of a chemiluminescence assay and an ELISA for detection of anti-GBM antibodies. Ren Fail. 2020;42(1):48-53. doi: 10.1080/0886022X.2019.1702056.
14. Hayrapetyan H., Tran T., Tellez-Corrales E., Madiraju C. Enzyme-Linked Immunosorbent Assay: Types and Applications. Methods Mol Biol. 2023;2612:1-17. doi: 10.1007/978-1-0716-2903-1_1.
15. Kohl T.O., Ascoli C.A. Indirect Immunometric ELISA. Cold Spring Harb Protoc. 2017 May 1;2017(5). doi: 10.1101/pdb.prot093708.
16. Li L., Qiao S., Liu J., Zhou Y., Tong W., Dong S., Liu C., Jiang Y., Guo Z., Zheng H., Zhao R., Tong G., Li G., Gao F. A highly efficient indirect ELISA and monoclonal antibody established against African swine fever virus pK205R. Front Immunol. 2023;13:1103166. doi: 10.3389/fimmu.2022.1103166.
17. Li J., Cao Y., Wang M., Cheng A., Ou X., Mao S., Sun D., Liu M., Zhang S., Zhao X.X., Jia R., Yang Q., Wu Y., Zhu D., Chen S., Huang J., Gao Q., Tian B. Development of an indirect ELISA method based on the VP4 protein for detection antibody against duck hepatitis A virus type 1. J Virol Methods. 2022;300:114393. doi: 10.1016/j.jviromet.2021.114393.
18. Cheng T.Y., Magtoto R., Henao-Díaz A., Poonsuk K., Buckley A., Van Geelen A., Lager K., Zimmerman J., Giménez-Lirola L. Detection of pseudorabies virus antibody in swine serum and oral fluid specimens using a recombinant gE glycoprotein dual-matrix indirect ELISA. J Vet Diagn Invest. 2021;33(6): 1106-114. doi: 10.1177/10406387211040755.
Review
For citations:
Laiqing L., Cuicui Ch., Huankun L., Zhong Sh., Leontiev V.N., Voitov I.V. Establishment of an indirect ELISA method for detecting canine distemper virus antibodies. Ecology and Animal World. 2024;(1):59-64.
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