Methods of decontamination of cell lines from mycoplasmas and acholeplasmas using antibacterial drugs
Abstract
The article presents experimental data on the development and evaluation of the effectiveness of decontamination schemes for transplanted cell cultures from Mollicutes class bacteria (mycoplasma and acholeplasma). The efficacy of three antimicrobial regimens, commercial Plasmocin and Plasmocure, as well as the combination of tylosin and doxycycline, was compared in mycoplasma-contaminated EPS and 3KG cell lines. The high efficiency of all schemes in their adaptation to specific cultivation conditions has been established. Mechanisms of action of applied antibiotics and their potential activity against mycoplasmas and acholeplasmas, considering common targets in prokaryotic cells, are considered. Principles and algorithm of actions for development of effective decontamination schemes are formulated.
Keywords
decontamination,
cell culture,
mycoplasmas,
acholeplasmas,
antibiotics,
Plasmocin,
Plasmocure,
tylosin,
doxycycline,
PCR.
About the Authors
Yu. I. Tsiapsha
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
I. I. Strealchenia
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Belarus
Belarus
A. V. Dubanevich
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
A. S. Andrusevich
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
O. V. Cherevako
РУП «Институт экспериментальной ветеринарии им. С.Н. Вышелесского»
Russian Federation
Russian Federation
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Review
For citations:
Tsiapsha Yu.I., Strealchenia I.I., Dubanevich A.V., Andrusevich A.S., Cherevako O.V. Methods of decontamination of cell lines from mycoplasmas and acholeplasmas using antibacterial drugs. Ecology and Animal World. 2025;(2):33-38. (In Russ.)
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